For Research Use Only — Vector-Control Surveillance

Pro-AmpRT™ WNV Isothermal Kit — In-House West Nile Detection in Under 30 Minutes

LAMP-based RNA detection on the Optigene Genie II/III. Closed-tube system, 30-min amplification at 66 °C, anneal-curve confirmation. Step 3 of the ProAmpRT™ in-house arbovirus surveillance system.

check_circle 100 Reactions Per Kit check_circle 30-Min Amplification @ 66 °C check_circle Anneal Confirmation 86.4–88.4 °C
Pro-AmpRT WNV Isothermal Kit PLM-1008 — reagent vials, Optigene 8-tube strip, and OP-0008 Genie Strips box for LAMP-based West Nile Virus detection
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Independent Validation

Burkhalter et al., J Med Entomol 2018 (PMID: 29462341)

science100 Reactions / Kit
timer30-Min Amplification
menu_bookIndependent Validation
securityResearch Use Only

From Extracted RNA to Result — In-House, Same Day

PLM-1008 is the LAMP detection step. Per the IFU procedure:

01
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Prepare Amp Mix

15 µL Master Mix + 4.975 µL WNV_E Primer Mix + 0.025 µL Reverse Transcriptase per reaction. Scale for run + 2 extra. Aliquot 20 µL into each Optigene 8-tube strip well.

02
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Add Sample

Add 5 µL of extracted RNA to each tube and cap immediately. Total reaction volume: 25 µL. Use a template-addition area separate from pre-amp setup to control contamination.

03
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Run on Genie II/III

Amplification: 66 °C × 30 min. Annealing: 98 °C → 80 °C at 0.05 °C/sec. Examine amplification curves and the anneal peak (WNV-positive: 86.4–88.4 °C).

Source: PLM-1008 IFU Ver: 160601

Why Vector-Control Programs Choose ProAmpRT WNV

Closed-Tube LAMP

Isothermal Amplification at 66 °C

No thermal cycling. Tubes stay closed after the run — per the IFU, "do not open after the run" to prevent amplicon contamination.

Anneal Confirmation

WNV Peak 86.4–88.4 °C

Built-in confirmation step. The Genie ramps from 98 °C to 80 °C at 0.05 °C/sec; the anneal peak distinguishes a true WNV-positive from amplification artifacts.

Independent Study

LAMP Detected What RAMP Missed (Honey Cards)

Burkhalter et al. (2018) compared ProAmpRT LAMP, RAMP, and RT-PCR on honey cards; ProAmpRT detected WNV/ZIKV at multiple titers where RAMP returned no positives.

Integrated Workflow

PLM-2008 → PLM-2007 → PLM-1008

Single-vendor accountability. Pro-Lab supplies homogenization, RNA extraction, and LAMP detection — full IFU set, one tech-support contact.

Designed for Your Role

Same-Day In-House WNV Detection — Closed-Tube LAMP, 30 Minutes on the Genie

If you ship pools to the state lab, the rhythm is the same every season — submit Monday, hope by Thursday, and during peak season the cap may bounce your submissions back. PLM-1008 closes the in-house loop. Add 5 µL of extracted RNA to a tube of amplification mix, run 30 minutes at 66 °C on the Optigene Genie II/III, read the anneal peak (86.4–88.4 °C confirms WNV-positive). Same-day surveillance result, every pool, every day.

  • check_circle30-min amplification at 66 °C on Genie II/III — same-day surveillance result
  • check_circleNo state-lab volume cap when surveillance runs in-house
  • check_circleIndependent honey-card evaluation: LAMP detected what RAMP missed (Burkhalter 2018; PMID: 29462341)

Closed-Tube LAMP — 25 µL Per Reaction, 30 Minutes, Anneal Confirmation

Setup is the same every run. Thaw primer mix and master mix on ice. Combine: 15 µL Master Mix + 4.975 µL WNV_E Primer Mix + 0.025 µL Reverse Transcriptase = 20 µL amp mix; +5 µL of extracted RNA = 25 µL total. Run on Genie II/III: 66 °C × 30 min, then anneal 98 °C → 80 °C at 0.05 °C/sec. Read both the amplification curve and the anneal peak. Closed-tube — do not open after the run.

  • check_circleReagents Provided: WNV_E Primer Mix (PLM-1008), Master Mix (PLM-501), Reverse Transcriptase (PLM-500)
  • check_circleUser-supplied: 1.5 mL RNase/DNase-free tubes, filtered tips, mini centrifuge, Optigene 8-tube strips (OP-0008), Genie II/III, cooled tube-strip holder
  • check_circleClosed-tube format reduces amplicon-contamination risk

Documented, Independently Evaluated, RUO — Surveillance Your Program Can Defend

Public-health labs need three things to adopt a surveillance method: the protocol in writing, an independent assessment, and a vendor that supplies the entire workflow. PLM-1008 has all three. The IFU specifies amplification at 66 °C × 30 min, annealing 98 °C → 80 °C at 0.05 °C/sec, and an anneal peak at 86.4–88.4 °C as the WNV-positive signal. Burkhalter et al. (2018) compared ProAmpRT LAMP, RAMP, and real-time RT-PCR on honey-card specimens; LAMP detected WNV/ZIKV samples that RAMP returned as negative. For Research Use Only — vector-control surveillance, not clinical patient diagnosis.

  • check_circlePublished IFU (Ver: 160601) — verbatim protocol, anneal range, contamination controls
  • check_circleIndependent peer-reviewed evaluation: Burkhalter 2018, PMID: 29462341
  • check_circleFull upstream IFU coverage: PLM-2008 (homogenization), PLM-2004 / PLM-2000 (extraction)

Complete the ProAmpRT™ Workflow

PLM-1008 is the detection step. Pair it with upstream homogenization and RNA extraction from the same manufacturer.

PLM-2008
Step 1 · Homogenization

Mosquito Collection & Homogenization Kit

Pre-filled 2.0 mL tubes with bearing balls and Grinding Buffer. Pools n ≤ 50, 100 preps per kit.

Details
PLM-2007
Step 2 · Automated

Mag Pro Plus Automated RNA Extraction Kit

Pre-loaded extraction plates designed for the Mag Pro-32 instrument — 32 samples per run.

Details
PLM-NPA-32
Instrument · Step 2

Mag Pro-32 Extraction Instrument

Automated 32-sample magnetic-bead extraction with built-in UV decontamination.

Details
PLM-2004
Step 2 · Spin Column

Pure Pro-Spin™ RNA Extraction Kit

Silica spin-column RNA extraction with published mosquito protocol. Manual workflow — no instrument required.

Details
PLM-2000
Step 2 · Manual Magnet

Pro-Mag™ RNA Extraction Kit

Manual magnetic-bead RNA extraction with hand magnet. Flexible throughput; no instrument required.

Details
Genie II / III
Detection Reader

Optigene Genie II / Genie III

Required isothermal LAMP reader with amplification and annealing analysis. Specified by the PLM-1008 IFU.

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Technical Specifications

SpecificationValue
Catalog NumberPLM-1008
Product TypeLAMP Primer Mix Kit (RUO)
RUO Designation"Not for Clinical Use." For Research Use Only — vector-control surveillance, not clinical diagnosis
Reactions Per Kit100
Kit ContentsPro-AmpRT WNV_E Primer Mix (PLM-1008); Pro-AmpRT Master Mix (PLM-501); Pro-AmpRT Reverse Transcriptase (PLM-500)
Per-Reaction Setup15 µL Master Mix + 4.975 µL WNV_E Primer Mix + 0.025 µL Reverse Transcriptase = 20 µL amp mix; +5 µL sample = 25 µL total
Detection ReaderOptigene Genie II / Genie III
Tube StripsOptigene 8 Tube Strips (cat# OP-0008)
Amplification66 °C × 30 min
Annealing98 °C → 80 °C at 0.05 °C/sec
WNV Anneal Peak86.4 – 88.4 °C
Detection MethodFluorescence (closed-tube)
SpecimenExtracted RNA (from PLM-2008 → PLM-2007 / PLM-2004 / PLM-2000 upstream workflow)
Cold ShipRequired (manufacturer note)
ManufacturerPro-Lab Diagnostics USA

Specifications sourced from PLM-1008 IFU Ver: 160601. Storage / shelf life and confirmed GHS hazard classification pending receipt of PLM-1008 SDS from Pro-Lab.

Frequently Asked Questions

PLM-1008 is the LAMP-based detection step of the ProAmpRT arbovirus surveillance system. It contains LAMP primers, master mix, and reverse transcriptase for closed-tube isothermal amplification of West Nile Virus RNA on the Optigene Genie II/III. 100 reactions per kit. For Research Use Only.
30-minute amplification at 66 °C plus annealing analysis (98 °C → 80 °C at 0.05 °C/sec). Source: PLM-1008 IFU Ver: 160601.
An anneal peak in the 86.4–88.4 °C range confirms WNV-positive. The Genie ramps from 98 °C to 80 °C at 0.05 °C/sec after amplification.
An Optigene Genie II or Genie III isothermal reader. Optigene 8-tube strips (cat# OP-0008) are required consumables. The product page references generic qPCR instrumentation — confirm specific qPCR-platform compatibility with Pro-Lab tech support.
No. The IFU header specifies "Not for Clinical Use." ProAmpRT is a Research Use Only system intended for vector-control and public-health surveillance — not for clinical patient diagnosis. Clinical patient diagnosis remains the role of cleared IVD assays at clinical reference labs.
Extracted RNA from the upstream ProAmpRT workflow: mosquito-pool homogenization (PLM-2008) → RNA extraction (PLM-2007 automated, PLM-2004 spin-column, or PLM-2000 manual magnet) → LAMP detection (PLM-1008).
LAMP is RNA-based; RAMP is antigen-based. An independent honey-card evaluation (Burkhalter et al., J Med Entomol. 2018; PMID: 29462341) found ProAmpRT LAMP detected WNV/ZIKV samples that RAMP returned as negative. ProAmpRT also covers EEE, WEE, SLE, LAC, and CHIKV via separate kits — RAMP does not.
Controls are not included. The IFU notes positive and negative controls are "advisable in each run." Establish your own positive (commercial WNV RNA standard) and no-template negative per your internal QC SOP.

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PLM-1008 · 100 reactions per kit · ProAmpRT consumables.

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Vector-control applications · (512) 789-5214

For Research Use Only — Not for Use in Diagnostic Procedures. The Pro-AmpRT WNV Isothermal Kit (PLM-1008) is designated Research Use Only per the IFU header (Ver: 160601 — verbatim "Not for Clinical Use"). The system is intended for vector-control and public-health surveillance — not for clinical patient diagnosis. The Burkhalter et al. (J Med Entomol. 2018; PMID: 29462341) study evaluated honey-card specimens, not mosquito-pool homogenate; performance in mosquito pools may differ. The "30-min amplification at 66 °C" run condition is verbatim from the PLM-1008 IFU Ver: 160601.