For Research Use Only — Vector-Control Surveillance

Mosquito Collection & Homogenization Kit — In-House Arbovirus Surveillance Starts Here

Pre-filled 2.0 mL tubes with bearing balls and Grinding Buffer — step 1 in the ProAmpRT™ system that delivers WNV, Dengue, and Zika surveillance results in under 30 minutes.

check_circle 100 Preps Per Kit check_circle Pools up to 50 Mosquitoes check_circle 12-Month Shelf Life
PLM-2008 Mosquito Collection and Homogenization Kit — 2.0 mL pre-filled tubes with bearing balls and grinding buffer for arbovirus surveillance
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Independent Validation

Burkhalter et al., J Med Entomol 2018 (PMID: 29462341)

science 100 Preps / Kit
timer Under 30-Min Workflow
menu_book Published Validation
security Research Use Only

From Pool to Result — In-House, Same Day

Three steps replace the 1–5 day state lab wait. PLM-2008 is the starting point.

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Homogenize

Add up to 50 mosquitoes to a pre-filled PLM-2008 tube. Bead-beat 35 sec at 4,000 rpm × 2. Centrifuge 5–10 min at 12,000 × g. Transfer 200 µL of clarified supernatant to extraction.

02
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Extract RNA

Choose your throughput: PLM-2007 (Mag Pro Plus, automated on the Mag Pro-32), PLM-2004 (Pure Pro-Spin, silica spin column), or PLM-2000 (Pro-Mag, manual magnetic bead).

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Detect by LAMP

PLM-1008 (WNV) or other ProAmpRT LAMP kits — 30-minute amplification at 66 °C on the Optigene Genie II/III isothermal reader. Same-day surveillance result.

Why Vector-Control Programs Choose ProAmpRT

Pre-Filled Tubes

No Buffer Prep, No Lot-to-Lot Variability

Each 2.0 mL tube ships pre-filled with a bearing ball and Grinding Buffer. No weighing, no pipetting, no prep step between technicians.

100 Preps / Kit

Full-Season Throughput in One Box

100 homogenization preparations — enough for a full surveillance season for most small-to-mid-size MADs and vector-control labs.

Same-Day Workflow

Under 30 Minutes — No State Lab Queue

PLM-2008 → RNA extraction → LAMP detection in under 30 minutes (manufacturer-stated). Replaces 1–5 day state lab TAT.

Independent Study

LAMP Detected What RAMP Missed

In a published evaluation on honey cards, ProAmpRT LAMP detected WNV/ZIKV samples that RAMP returned as negative (Burkhalter et al., 2018; PMID: 29462341).

Designed for Your Role

Stop Waiting Days for West Nile Results — Test In-House, Spray Same Day

Most districts send pools to the state lab and wait — sometimes 3–5 days, with weekly volume caps. ProAmpRT delivers WNV, Dengue, and Zika surveillance results in under 30 minutes, with no submission cap and no state lab queue. PLM-2008 is the first step — pre-filled tubes for pools up to 50 mosquitoes.

  • check_circleSame-day surveillance result enables same-day spray decisions
  • check_circleNo state-lab volume cap when you screen in-house
  • check_circleCovers 8 arboviruses on one platform — WNV, DENV, ZIKV, CHIKV, EEE, WEE, SLE, LAC

Consistent Mosquito Pool Homogenization — No Buffer Prep, No Lot Variability

DIY grinding buffer is consistent until it isn't. PLM-2008 ships pre-filled with the same bearing ball and Grinding Buffer in every tube — manufactured under lot control. Bead-beat 35 sec at 4,000 rpm × 2, centrifuge, transfer 200 µL. Same result every run, regardless of who's at the bench.

  • check_circlePre-filled, lot-controlled — no weighing, no pipetting
  • check_circleStandard lab storage — RT, 12-month shelf life (Grinding Buffer 2–8°C)
  • check_circleWorks with bead mixer or rotor-stator homogenizer — no proprietary instrument

Published Arbovirus Surveillance Workflow — Independent, Documented, RUO

ProAmpRT LAMP has been independently evaluated and published in the Journal of Medical Entomology (Burkhalter et al., 2018; PMID: 29462341). PLM-2008 provides lot-controlled, IFU-documented homogenization reagents — a traceable record from collection to surveillance result. (For Research Use Only — not for clinical patient diagnosis.)

  • check_circlePeer-reviewed independent validation (CDC + UFL researchers)
  • check_circleFull IFU documentation: PLM-1008, PLM-2004, PLM-2000
  • check_circleNNDSS-reportable arboviruses: WNV, Dengue, Zika, EEE, WEE, SLE, LAC

Complete the ProAmpRT™ Workflow

PLM-2008 is step 1. Pair it with downstream RNA extraction and LAMP detection from the same manufacturer.

PLM-2007
Step 2 · Automated

Mag Pro Plus Automated RNA Extraction Kit

Pre-loaded extraction plates designed for the Mag Pro-32 instrument — 32 samples per run, 15–40 minutes.

Details
PLM-1008
Step 3 · LAMP Detection

Pro-AmpRT™ WNV Isothermal Kit

LAMP-based West Nile Virus detection. 30-min amplification at 66 °C on Optigene Genie II/III; WNV anneal peak 86.4–88.4 °C.

Details
PLM-NPA-32
Instrument · Step 2

Mag Pro-32 Extraction Instrument

Automated 32-sample magnetic-bead extraction with built-in UV decontamination. Walk-away protocol.

Details
PLM-2004
Step 2 · Spin Column

Pure Pro-Spin™ RNA Extraction Kit

Silica spin-column RNA extraction with published mosquito protocol. 50 reactions per kit. Manual workflow — no instrument required.

Details
PLM-2000
Step 2 · Manual Magnet

Pro-Mag™ RNA Extraction Kit

Manual magnetic-bead RNA extraction with hand-magnet rack. Flexible throughput; pairs with PLM-2008 homogenate.

Details
Genie II / III
Detection Reader

Optigene Genie II / Genie III

Isothermal LAMP reader with amplification and annealing analysis. Required instrument for the PLM-1008 LAMP detection step.

Learn More

Technical Specifications

Specification Value
Catalog NumberPLM-2008
Product TypePre-filled mosquito pool homogenization consumable
RUO DesignationFor Research Use Only — Not for Use in Diagnostic Procedures
Contents2.0 mL tubes pre-filled with bearing balls (BB) and Grinding Buffer
Preparations Per Kit100
Maximum Pool Size50 mosquitoes per pool
Specimen TypeMosquito whole-body pools (kept frozen prior to processing)
Homogenization StepBead mixer or rotor-stator homogenizer; 35 sec at 4,000 rpm × 2
ClarificationCentrifuge 5–10 min at 12,000 × g (room temperature)
Transfer Volume200 µL clarified supernatant to RNA extraction
StorageRoom temperature; Grinding Buffer 2–8°C; do not freeze
Shelf LifeAt least 12 months under correct storage
WorkspaceClass II Biosafety Cabinet recommended
Compatible RNA ExtractionPLM-2007 (automated), PLM-2004 (spin column), PLM-2000 (manual magnet)
Compatible LAMP DetectionPLM-1008 (WNV), PLM-1005 (DENV 1–4), additional ProAmpRT kits for ZIKV/CHIKV/EEE/WEE/SLE/LAC
Detection ReaderOptigene Genie II / Genie III
ManufacturerPro-Lab Diagnostics

Specifications sourced from PLM-2004 IFU (Ver: 20180706) mosquito protocol and PLM-1008 IFU (Ver: 160601). Contact Pro-Lab Direct for the PLM-2008 standalone IFU and SDS.

Frequently Asked Questions

PLM-2008 is a sample-preparation kit containing 2.0 mL tubes pre-filled with bearing balls and Grinding Buffer for standardized mosquito pool homogenization. It is step 1 in the ProAmpRT arbovirus surveillance workflow. Each kit provides 100 preparations.
Up to 50 mosquitoes per pool. Per the Pro-Lab mosquito protocol IFU (PLM-2004 Ver: 20180706): mosquito pools (n ≤ 50) should be collected into 2.0 mL tubes and kept frozen prior to processing to prevent RNA degradation.
A bead mixer or rotor-stator homogenizer (user-supplied). Per the Pro-Lab mosquito protocol IFU: homogenize 35 seconds at 4,000 rpm, twice; centrifuge 5–10 minutes at 12,000 × g at room temperature; transfer 200 µL of clarified supernatant to the downstream RNA extraction step.
The kit is stored at room temperature with one exception: the Grinding Buffer should be stored at 2–8°C. The kit is stable for at least 12 months under correct storage conditions. Do not freeze any kit components.
After homogenization with PLM-2008 and RNA extraction (PLM-2007 / PLM-2004 / PLM-2000), downstream ProAmpRT LAMP detection kits cover West Nile Virus (PLM-1008), Dengue serotypes 1–4 (PLM-1005), Zika, Chikungunya, Eastern Equine Encephalitis, Western Equine Encephalitis, St. Louis Encephalitis, and La Crosse Encephalitis.
LAMP detects viral RNA; RAMP detects viral antigen. An independent study (Burkhalter et al., J Med Entomol. 2018;55(3):717; PMID: 29462341) evaluated both platforms on honey cards and found LAMP detected WNV and Zika positive samples that RAMP returned as negative. ProAmpRT LAMP also covers EEE, WEE, SLE, LAC, and CHIKV — RAMP does not.
A bead mixer or rotor-stator homogenizer for PLM-2008; a microcentrifuge capable of >12,000 × g; for RNA extraction either the Mag Pro-32 instrument (PLM-NPA-32) for automated workflow or manual extraction with PLM-2004 (Pure Pro-Spin) or PLM-2000 (Pro-Mag); an Optigene Genie II or Genie III isothermal reader for LAMP detection (PLM-1008/1005); Optigene 8-tube strips (cat# OP-0008); standard lab consumables; and a Class II Biosafety Cabinet for handling live arbovirus material.
No. ProAmpRT — including PLM-2008, the RNA extraction kits, and the LAMP detection kits — is a Research Use Only (RUO) system intended for vector-control and public-health surveillance applications. The PLM-1008 IFU specifies "Not for Clinical Use." The RNA extraction IFUs state the products are "not intended for the diagnosis, prevention, or treatment of disease." Clinical patient diagnosis remains the role of cleared IVD assays in clinical reference labs.

Ready to Bring Arbovirus Surveillance In-House?

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Vector-control applications support · (512) 789-5214

For Research Use Only — Not for Use in Diagnostic Procedures. The Mosquito Collection and Homogenization Kit (PLM-2008) and the complete ProAmpRT™ workflow are designated Research Use Only (RUO) per applicable manufacturer regulatory designations. The PLM-1008 LAMP detection kit IFU specifies "Not for Clinical Use"; the PLM-2004 and PLM-2000 RNA extraction IFUs state the products are "not intended for the diagnosis, prevention, or treatment of disease." The system is intended for vector-control and public-health surveillance — not for clinical patient diagnosis. The "under 30 minutes" workflow time is manufacturer-stated for the complete ProAmpRT system. The Burkhalter et al. (J Med Entomol. 2018; PMID: 29462341) study evaluated honey-card specimens, not mosquito-pool homogenate; performance in mosquito pools may differ.