Name: DNase/RNase Free Water
Brand: Pro-Lab Diagnostics
SKU: PL.5902R

PCR Grade · PL.5903

80 × 2 mL Aliquot Box

Pre-portioned DNase/RNase-Free Water for PCR workflows that need small, ready-to-use volumes. Each box contains 80 × 2 mL aliquots of DEPC-free water, with per-lot DNase and RNase release testing.

$249

per 80 × 2 mL box

Why DEPC-Free Matters

DEPC (diethylpyrocarbonate) is the most common chemical treatment for nuclease removal — but DEPC residue is a documented inhibitor of downstream enzyme reactions, especially reverse transcription and in vitro translation. Most autoclave protocols are designed to convert DEPC to ethanol and CO₂, but trace residue is a recurring debate in molecular biology literature.

Pro-Lab's process removes nucleases without DEPC — designed for labs running sensitive RT-PCR, qPCR, IVT, or library-prep workflows where residual chemistry could matter.

Per-Lot Release Testing

Every lot is manufactured under controlled conditions and tested to confirm absence of detectable DNase and RNase activity. Testing uses validated enzymatic assays with sensitivity appropriate for trace nuclease detection. Only lots that meet internal release specifications are approved for distribution.

✓Per-lot DNase + RNase release testing — validated enzymatic assays
✓Lot # and expiry on every container — full traceability for CAP / CLIA / ISO 13485 documentation
✓Lot identified — lot number and expiry are printed on each container

Specs at a Glance

Catalog #

RUO: PL.5901R, PL.5902R, PL.5903R · PCR: PL.5901, PL.5902, PL.5903

Pack formats

25 mL bottle · 1 L bottle · 80 × 2 mL aliquot box

Storage

15–30 °C (room temperature)

DNase activity

Not detectable (per-lot release tested)

RNase activity

Not detectable (per-lot release tested)

DEPC treatment

None — DEPC-free process

Lot # + expiry

Printed on every container

CAS No.

7732-18-5

OSHA HCS classification

Not classified per 29 CFR 1910.1200

Hazard pictogram

None — not applicable

Grades

Research Use Only (RUO) and PCR Grade — per-lot release tested

Designed For

PCR · RT-PCR · qPCR

Master-mix prep, primer/probe dilution, no-template controls

Nucleic acid dilution

DNA / RNA stock dilution, library normalization

Enzyme reaction setup

Polymerases, reverse transcriptases, restriction enzymes, ligases

General mol-bio workflows

Any application requiring nuclease-free conditions

How It Compares

Spec	Pro-Lab PL.5901/5902	Thermo Ambion (AM99xx)	Invitrogen UltraPure (10977)	IDT (11-05-01-14)	In-House Milli-Q + DEPC
DEPC-free	Yes	No (DEPC-treated)	Yes	Yes	Usually DEPC-treated
Per-lot release testing	Yes (IFU)	Production-level	Per lot	Per lot	None
Pack format	1 L	50 mL → 100 mL	500 mL · 6×500 mL	50 mL · 1 L	DIY
Lot # + expiry on container	Yes	Yes	Yes	Yes	No
Factory direct	Yes — Texas	Distributor	Distributor	Yes — Iowa	n/a
CoA per lot	On request	Cert Tracker	On request	On request	None

Competitor data snapshot 2026-05; re-verified quarterly. Public catalogs + datasheets.

Pricing

RUO Grade

Research Use Only

25 mL bottle

PL.5901R

$49

1 L bottle

PL.5902R

$149

80 × 2 mL aliquot box

PL.5903R

$199

PCR Grade

PCR

25 mL bottle

PL.5901

$59

1 L bottle

PL.5902

$179

80 × 2 mL aliquot box

PL.5903

$249

Prices shown in USD. Shipping and applicable tax are calculated at checkout.

Compliance & Documentation

IFU — PL.5901/5902 product information sheet, revision 2026-05-1. Included with shipment.
SDS — DNase/RNase Free Water Safety Data Sheet, v1.0 (5/5/2026), conforms to OSHA HCS 29 CFR 1910.1200 (revised 2024).
Hazard status (US): Not classified per HCS — no pictogram, no signal word, no hazard or precautionary statements required.
Manufacturer: Pro-Lab Diagnostics, with ISO 13485 manufacturing.
Grade: Manufacturing-grade molecular biology water — built for production workflows, library prep, NGS, and QC labs.

Handling & Storage

Store at 15–30 °C (room temperature), in the original container, tightly closed.
Once opened, handle with nuclease-free technique to prevent environmental contamination.
Do not return used aliquots to the original container.
Do not use after the expiration date shown on the label.
Do not use if any signs of contamination are observed.
Avoid introduction of nucleases through improper handling.

FAQ

Is this water DEPC-treated?

No. Pro-Lab's process removes nucleases without DEPC or other chemical treatments.

Is every lot tested?

Yes — each lot is tested for absence of detectable DNase and RNase activity using validated enzymatic assays before release.

How should I store it?

15–30 °C (room temperature), in the original container, tightly closed and protected from contamination.

Is this suitable for production / manufacturing / NGS workflows?

Yes. This is manufacturing-grade molecular biology water — designed for production-scale PCR / RT-PCR / qPCR, NGS library prep, QC labs, and in-line reagent prep. Every lot is release-tested for DNase and RNase activity; lot number and expiry are printed on every container; CoA is available on request by lot.

What lot documentation is supplied?

The IFU confirms lot-release testing for DNase and RNase activity, and the lot number and expiry are printed on the container. Contact support@pro-lab.us to ask which additional controlled documents are available for a specific lot.

Do you offer volume pricing?

Yes — 6× and 12× case quantities are tiered. Use the Get Volume Quote button.

Do you ship internationally?

Contact Pro-Lab Direct for current US availability. For Canada and the UK, contact the applicable Pro-Lab regional office before ordering.

DNase/RNase Free Water — DEPC-Free, Per-Lot Release Tested

Available Formats